Absolute quantitation of microbiota abundance in environmental samples
نویسندگان
چکیده
منابع مشابه
Absolute quantitation of specific mRNAs in cell and tissue samples by comparative PCR.
A comparative PCR assay, for the absolute quantitation of specific mRNAs in cell and tissue samples, has been designed to overcome problems with previous techniques. cDNAs made from the RNAs are co-amplified with "competitor" plasmid templates under conditions in which reagents are not limiting at the equivalence point, thereby preventing competition between target and competitor templates and ...
متن کاملRelative Quantitation of Protein Abundance Relative Quantitation of Posttranslational Modifications
Sample Preparation for the Relative Quantitation of Protein Abundance Sample Preparation for Monitoring Components of a Protein Complex Sample Preparation for Relative Quantitation of Posttranslational Modifications Sample Preparation for the Identification of Tyrosine Kinase Substrates Electrophoresis and Silver Staining of Polyacrylamide Gels In-Gel Trypsin Digestion Reversed Phase Column Pre...
متن کاملAbsolute quantitation of post-translational modifications
The cell’s fate is largely governed by the dynamics of cellular events, brought about by the interplay of various proteins, in which post-translational modifications (PTMs) play a key role. Therefore, a better understanding of post-translational modification is of utmost importance to a biochemist. Over the years much work in this regard has been achieved through mass spectrometry, however, the...
متن کاملthe effect of taftan pozzolan on the compressive strength of concrete in the environmental conditions of oman sea (chabahar port)
cement is an essential ingredient in the concrete buildings. for production of cement considerable amount of fossil fuel and electrical energy is consumed. on the other hand for generating one tone of portland cement, nearly one ton of carbon dioxide is released. it shows that 7 percent of the total released carbon dioxide in the world relates to the cement industry. considering ecological issu...
Absolute Quantification of Endogenous Ras Isoform Abundance
Ras proteins are important signalling hubs situated near the top of networks controlling cell proliferation, differentiation and survival. Three almost identical isoforms, HRAS, KRAS and NRAS, are ubiquitously expressed yet have differing biological and oncogenic properties. In order to help understand the relative biological contributions of each isoform we have optimised a quantitative proteo...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Microbiome
سال: 2018
ISSN: 2049-2618
DOI: 10.1186/s40168-018-0491-7